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The Genetic Perturbation Platform formerly known as the RNA

The Genetic Perturbation Platform formerly known as the RNA

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Genetic Perturbation

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rna-seq

Approaches to genetic perturbation: shRNA knockdown, Cas9 knockout, and dCas9 transcriptional activation.

Single-cell and multivariate approaches in genetic perturbation screens | Nature Reviews Genetics

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Transcriptional repression via steric hindrance. CRISPR interference (CRISPRi) is a genetic perturbation ...

Editing false positives from cancer dependency maps drawn with CRISPR

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What combinations of mutations help cancer cells survive? Which cells in the brain are involved in the onset of Alzheimer's? How do immune cells conduct ...

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... Doench (Associate Director of the #Genetic #Perturbation #Platform, #BroadInstitute) on Tuesday, May 15/11:00 a.m. at MEM Murtenstrasse 35, room H813.

Different RNAi reagents targeting the same gene rarely produce similar profiles, whereas

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Figure 1: Screening GWAS genes identifies critical negative regulatory nodes controlling macrophage oxidative burst.

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Systematic discovery of genes and pathways regulating sensitivity and resistance of tumor

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Functional variomics and network perturbation: connecting genotype to phenotype in cancer | Nature Reviews Genetics

(A) Global transcriptional network perturbations were observed across cancer types. Global lncRNA modulators that mediated the network perturbations were ...

Download figure · Open in new tab · Download powerpoint. Figure EV1. Functional genetic perturbation of ...

Effects of mismatches in positions 9–11 on RNA and protein levels

Today Insilico Medicine announced the publication of its in silico Pathway Activation Network Decomposition Analysis (iPANDA), a novel approach for ...

A possible workaround for targeting the untargetable in a childhood ne.

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Schematic of the LCA That Determines Genes Mediating Certain Cellular Responses from Expression Profiles after Perturbation

Fig. 4. Enhanced responsiveness to IFNγ stimulation by Arid2 and Pbrm1 deficient tumor cells

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BioMedical Research

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Functional variomics and network perturbation: connecting genotype to phenotype in cancer | Nature Reviews Genetics

The ...

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... Download full-size image. Figure S5. Characteristics of the Genetic Perturbation ...

Five questions for David Root: RNA Interference explained

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Comparison of different sets of conditions: genetic perturbations (red), environmental perturbations (

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A single gene network accurately predicts phenotypic effects of gene perturbation in Caenorhabditis elegans | Nature Genetics

The CRISPR platform for gene editing, genomic regulation and imaging.

Large variation generated by an individual sgRNA can be used to select for

Functional variomics and network perturbation: connecting genotype to phenotype in cancer | Nature Reviews Genetics

Figure 2: CRISPR-based technology is commonly used to knock out specific genes (

Widespread lncRNA-mediated transcriptional regulation perturbations in 20 types of cancer. (A)

Combinatorial CRISPR–Cas9 screens for de novo mapping of genetic interactions | Nature Methods

With rapid advancements in gene editing tools like Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and single-cell analysis, genomics can ...

Validation of gain-of-function MAP2K1 alleles. A375 cells constitutively

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Diagream: Using CRISPR to create Indels

a | Three theoretical single-cell features are shown: the amount of an organelle such as the lysosome, the amount of epithelial growth factor receptor ...

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(A) RNA editing at non-coding RNAs, such as lncRNAs, miRNAs. (B) Modulation of gene expression in a spatial-temporal manner and epigenetic regulation using ...

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... 8. Is Cpf1 the ...

In the standard approach (top portion of figure) genes are analyzed for fold-change and p-value (statistical analysis) in order to identify differentially ...

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a, Candidate genes based on FDR cut-off of 0.1% (n = 554 genes) were functionally categorized by multiple methods to understand the biological significance ...

(a) The number of perturbed lncRNAs, protein-coding genes and microRNAs in human and mouse. (b) The number of perturbation datasets involved in primary cell ...

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Screening of a pooled sgRNA library tiling BRAF. (A) Comparison

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Differential expression analysis for individual cancer samples based on robust within-sample relative gene expression orderings across multiple profiling ...

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Determination of protein stability by the pAd-DsRed-IRES-EGFP-X reporter system coupled with CRISPR-Cas9 gene knockout.

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Results

FIGURE 2. Imperfectly complementary siRNAs result in degradation products that corre- spond to canonical

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Applications of GPA transcriptome analysis results mainly aim at detecting (i, green) novel or cell-specific functions and pathways affected by perturbed ...

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